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KMID : 1134819980270020319
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Journal of the Korean Society of Food Science and Nutrition
1998 Volume.27 No. 2 p.319 ~ p.325
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Effect of Green Tea Catechin on the Microsomal Mixed Function Oxidase System of Kidney and Brain in Streptozotocin - Induced Diabetic Rats
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Rhee Soon-Jae
Shin Ju-Young
Cha Bok-Kyeong
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Abstract
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The purpose of this study was to investigate the effect of green tea catechin on microsomal mixed function oxidase(MFO) system of kidney and brain in streptozotocin(STZ) induced diabetic rats. Sprague-Dawley male rats weighing 140±10g were randomly assigned to one control and three STZ-diabetic groups. Diabetic groups were classified to DM-0C(catechin 0%/㎏ diet), DM-0.5C(catechin 0.5%/㎏ diet), and DM-1.0C(catechin l%/㎏ diet) according to the level of catechin supplementation. Diabetes were experimentally induced by intravenous administration of 55㎎/㎏ body weight of STZ in citrate buffer(pH 4.3) after 4 weeks feeding of three experimental diets. Animals were sacrificed at the sixth day of diabetic state. The contents of cytochrome P_(450) in kidney were increased by 77, 42, 49% in DM-0C, DM-0.5C and DM-1.0C groups, respectively, than normal group. The contents of cytochrome P_(450) in brain were increased by 43% in DM-0C group than normal group, but those of DM-0.5C and DM-1.0C groups were similar to that of normal group. The contents of cytochrome b_5 in kidney were increased by 78, 38, 49% in DM-0C, DM-0.5C and DM-1.0C groups, respectively, than normal group. Meanwhile, the contents of cytochrome b_5 in brain were not significantly different among all groups. The activities of NADPH-cytochrome P_(450) reductase in kidney of DM-group were increased by 27% than normal group, but those of DM-0.5C and DM-1.0C groups were 13 and 15% lower than that of DM-0C group. The activities in brain were also increased by 31% in DM-0C group, but those of DM-0.5C and DM-1.0C groups were similar to that of normal group. Levels of TBARS(thiobarbituric acid reactive substance) in kidney were increased by 147, 60 and 59% in DM-0C, DM-0.5C, and DM-1.0C groups, respectively, compared with normal group, but those of DM-0.5C and DM-1.0C groups were 36, 35% lower than that of DM-0C group. Meanwhile, the levels of TBARS in brain were not significantly different among four groups. These results indicate that dietary catechins in green tea playa powerful antioxidant role in reducing the lipid peroxidation enhanced by activation of MFO system in STZ-induced diabetes.
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KEYWORD
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diabetes, mixed function oxidase system, catechin
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